Review Team (February 2016)

BVit-03 (February 2016) FOR ERP USE ONLY DO NOT DISTRIBUTE

AOAC- SPIFAN-2012-VITAMINS

Validation Parameter

Limit of Quantification

Test Group Name

Date

Prasad BKVR/Kesav Kumar N TUV/BLR/F-360 TUV/BLR/F-99 Manjunath K/Prasad BKVR 01.01.2015 2015\January\Vitamins\01.01.2015

B-Complex vitamins Riboflavin Aqueous

Test Name

Data Path File

Matrix

Raw-Data File Ref. CRM Data File Ref.

Limit

Equipment Test Method

Analyst

LC-MS/MS 6460A (BLR/PS-CRS/006.03)

Operator

Repeatability at LOQ 5 ug/l standard Sample Blank No.

Mean Retention Time (min)

m/z

Signal (ug/l)

Caluclation

377.1 >243.2 377.1 >243.2 377.1 >243.2 377.1 >243.2 377.1 >243.2 377.1 >243.2 377.1 >243.2 377.1 >243.2 377.1 >243.2 377.1 >243.2

3.941 3.941 3.941 3.941 3.941 3.941 3.941 3.941 3.941 3.941

5.465 5.462 5.257 5.358 5.192 5.201 5.439 5.537 5.300 5.312

1 2 3 4 5 6 7 8 9

Mean (μg/l)

5.35

SD

0.1195

LOD (Mean +3 sigma) LOQ (Mean + 10sigma)

5.71 6.55 2.23

RSD %

5

Standard Conc. (μg/l)

107.0

Accuracy %

Accuracy = 70 - 125 %

Criteria

10

RSD% = <15%

Page 22