SPADA

Significance • Is brucellosis truly eradicated in the United  States? • Is B. suis distinguishable from B. canis ? • Do feral swine pose a greater risk to human and  animal health than Yellowstone bison and elk? • Does our working group feel we can address the  standard method performance requirements for  B. suis  real‐time PCR assays, as well as for B.  abortus and B. melitensis ?

General Analytical Needs • Working group tasked with addressing B suis .  • Working group to decide if B. abortus and B.  melitensis  can be addressed at the same time • Detection in aerosols (air collection of particulates  onto filters) I l i l tbd • nc us on pane s ‐ • Exclusion panels – tbd, but also a common SMPR  panel that has been established from air sampling  experiences (eg BioWatch) • Chemical contaminant background

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