SPDS SET 2: ASH-01

NATURAL REMEDIES PVT LIMITED QUALITY CONTROL DEPARTMENT

Acquired by Sample Name

: Admin

: Withania somnifera : WS / 06 lot 10

Sample ID

: 2000 mg / 100 ml : Trl 1

Vail #

: 36

Injection Volume Data File Name Method File Name Batch File Name Report File Name Data Acquired Data Processed

: 20 uL

: Rep-RP007.lcd : Withaina.met.lcm : Batch 01.lcb : Calibration.lcr

: 1/16/2007 6:07:54 AM : 1/23/2007 3:15:45 AM

D:\LC2010AHT\2007\Method validation\Withania somnifera\Jan 2007\Rep-RP007.lcd

mV

1Det.A Ch1

500

3

400

300

1

4

200

5

2

100

6

0

0

5

10

15

20

25

30

35

40

45

min

Det.A Ch1/227nm 1

Detector A Ch1 227nm Peak # Ret. Time

Area 1220186 1028111 2795043 1401887 983304 314770 7743301

Height

Area %

Name

1 2 3 4 5 6 Total

15.548 19.717 20.388 21.391 22.248 25.530

215604 147371 395277 231397 177119 56268 1223036

15.758 13.277 36.096 18.105 12.699 4.065 100.000

Withanoside IV Withanoside V Withaferin A 12- Deoxy withastramonolide

Withanollide A Withanolide B

D:\LC2010AHT\2007\Method validation\Withania somnifera\Jan 2007\Rep-RP007.lcd