SPDS SET 2: ASH-01

NATURAL REMEDIES PVT LIMITED QUALITY CONTROL DEPARTMENT

Acquired by Sample Name

: Admin

: Withania somnifera ext

Sample ID

: WS - RD / 1170

Vail #

: 16

Injection Volume Data File Name Method File Name Batch File Name Report File Name Data Acquired Data Processed

: 20 uL

: Quan003.lcd

: Withaina.met.lcm : Batch 01.lcb : Calibration.lcr

: 1/15/2007 9:32:37 AM : 1/23/2007 2:48:46 AM

D:\LC2010AHT\2007\Method validation\Withania somnifera\Jan 2007\Quan003.lcd

mV

1Det.A Ch1

400

3

300

4

1

5

200

2

100

6

0

0

5

10

15

20

25

30

35

40

45

min

Det.A Ch1/227nm 1

Detector A Ch1 227nm Peak # Ret. Time

Area

Height

Area %

Name

1 2 3 4 5 6 Total

15.526 19.689 20.361 21.381 22.238 25.532

941120 804822 2646818 1480793 933574 320629 7127757

166252 115826 369982 230122 169341 57353 1108877

13.204 11.291 37.134 20.775 13.098 4.498 100.000

Withanoside IV Withanoside V Withaferin A 12- Deoxy withastramonolide

Withanollide A Withanolide B

D:\LC2010AHT\2007\Method validation\Withania somnifera\Jan 2007\Quan003.lcd