SPDS SET 2: ASH-01

NATURAL REMEDIES PVT LIMITED QUALITY CONTROL DEPARTMENT

Acquired by Sample Name

: Admin

: Withania somnifera ext

Sample ID

: WS - 05 LOT 21

Vail #

: 22

Injection Volume Data File Name Method File Name Batch File Name Report File Name Data Acquired Data Processed

: 20 uL

: SR001.lcd

: Withaina.met.lcm : Batch 01.lcb : Calibration.lcr

: 1/15/2007 2:07:03 PM : 1/23/2007 3:21:56 AM

D:\LC2010AHT\2007\Method validation\Withania somnifera\Jan 2007\SR001.lcd

mV

1Det.A Ch1

400

3

300

4

5

200

6

1

2

100

0

0

5

10

15

20

25

30

35

40

45

min

Det.A Ch1/227nm 1

Detector A Ch1 227nm Peak # Ret. Time

Area

Height

Area %

Name

1 2 3 4 5 6 Total

15.537 19.685 20.360 21.369 22.232 25.520

783819 696402 2695778 1614288 1351576 1087909 8229773

137203 99709 385557 276366 233890 192244 1324969

9.524 8.462

Withanoside IV Withanoside V Withaferin A 12- Deoxy withastramonolide

32.756 19.615 16.423 13.219 100.000

Withanollide A Withanolide B

D:\LC2010AHT\2007\Method validation\Withania somnifera\Jan 2007\SR001.lcd