SPDS SET 2: ASH-01

NATURAL REMEDIES PVT LIMITED QUALITY CONTROL DEPARTMENT

Acquired by Sample Name

: Admin

: Withania somnifera ext

Sample ID

: WS - RD / 1170

Vail #

: 24

Injection Volume Data File Name Method File Name Batch File Name Report File Name Data Acquired Data Processed

: 20 uL

: SR003.lcd

: Withaina.met.lcm : Batch 01.lcb : Calibration.lcr

: 1/15/2007 3:38:30 PM : 1/23/2007 3:24:38 AM

D:\LC2010AHT\2007\Method validation\Withania somnifera\Jan 2007\SR003.lcd

1Det.A Ch1

3

400

4

300

5

6

200

1

2

100

0

0

5

10

15

20

25

30

35

40

45

min

Det.A Ch1/227nm

Detector A Ch1 227nm Peak # Ret. Time

Area

Height

Area %

Name

1 2 3 4 5 6 Total

15.519 19.682 20.354 21.369 22.230 25.525

944658 849034 3012010 2073431 1525217 1226660 9631011

165451 121893 429420 335816 270242 214413 1537235

9.809 8.816

Withanoside IV Withanoside V Withaferin A 12- Deoxy withastramonolide

31.274 21.529 15.837 12.737 100.000

Withanollide A Withanolide B

D:\LC2010AHT\2007\Method validation\Withania somnifera\Jan 2007\SR003.lcd