SPDS SET 2: ASH-01

QUALITY CONTROL DEPARTMENT

Name of Sample Method Name Data File Name

: Withania somnifera : WS - 06 lot 08

: C:\Methods\WITHANIA.met

: D:\LC10-ATvp\2007\Method validation\Apr 2007\020407\S3-Rep3

Vial No

: 10004

Injection Volume

: 20

Date & Time

: 4/4/07 6:26:43 AM

0.5

0.4

22.025

0.3

17.225

23.017

Volts

0.2

23.908

21.492

0.1

26.975

0.0

0

5

10

15

20

25

30

35

40

45

Minutes

Detector A - 1 (227nm)

Pk # Retention Time Name

Area Area Percent

1 2 3 4 5 6

17.225 21.492 22.025 23.017 23.908 26.975

Withanoside IV Withanoside V Withaferin A

1207529 808773 2109929 1186698 1089796 265589

18.11 12.13 31.64 17.80 16.34

Withastramonolide

Withanolide A Withanolide B

3.98

Totals

6668314

100.00