SPDS SET 2: ASH-01

QUALITY CONTROL DEPARTMENT

Name of Sample Method Name Data File Name

: Withania somnifera : WS - 06 lot 10

: C:\Methods\WITHANIA.met

: D:\LC10-ATvp\2007\Method validation\Apr 2007\020407\S4-Rep1

Vial No

: 10005

Injection Volume

: 20

Date & Time

: 4/4/07 7:12:29 AM

0.4

22.025

0.3

17.225

23.017

0.2

Volts

23.908

21.500

0.1

26.975

0.0

0

5

10

15

20

25

30

35

40

45

Minutes

Detector A - 1 (227nm)

Pk # Retention Time Name

Area Area Percent

1 2 3 4 5 6

17.225 21.500 22.025 23.017 23.908 26.975

Withanoside IV Withanoside V Withaferin A

1178151 770729 1928284 1102671 960958 249257

19.03 12.45 31.15 17.81 15.52

Withastramonolide

Withanolide A Withanolide B

4.03

Totals

6190050

100.00