SPDS SET 2: ASH-01

QUALITY CONTROL DEPARTMENT

Name of Sample Method Name Data File Name

: Withania somnifera : WS - 05 lot 21

: C:\Methods\WITHANIA.met

: D:\LC10-ATvp\2007\Method validation\Apr 2007\020407\S2-Rep2

Vial No

: 10003

Injection Volume

: 20

Date & Time

: 4/4/07 12:33:26 PM

0.25

0.20

0.15

17.217

0.10

Volts

23.025

23.900

21.408

0.05

27.017

0.00

22.050

0

5

10

15

20

25

30

35

40

45

Minutes

Detector A - 1 (227nm)

Pk # Retention Time Name

Area Area Percent

1 2 3 4 5 6

17.217 21.408 22.050 23.025 23.900 27.017

WIthanoside IV Withanoside V

599396 377387 1413249 502270 471080 100538

17.30 10.89 40.80 14.50 13.60

Withaferin A

Withastramonolide

Withanolide A Withanolide B

2.90

Totals

3463920

100.00