SPDS SET 2: ASH-01

QUALITY CONTROL DEPARTMENT

Name of Sample Method Name Data File Name

: Withania somnifera : WS - 05 lot 21

: C:\Methods\WITHANIA.met

: D:\LC10-ATvp\2007\Method validation\Apr 2007\020407\S2-Rep3

Vial No

: 10003

Injection Volume

: 20

Date & Time

: 4/4/07 1:19:18 PM

0.25

0.20

0.15

17.225

0.10

23.042

Volts

23.908

21.417

0.05

27.033

0.00

22.067

0

5

10

15

20

25

30

35

40

45

Minutes

Detector A - 1 (227nm)

Pk # Retention Time Name

Area Area Percent

1 2 3 4 5 6

17.225 21.417 22.067 23.042 23.908 27.033

WIthanoside IV Withanoside V

596170 378348 1422577 502808 473580 100847

17.16 10.89 40.95 14.47 13.63

Withaferin A

Withastramonolide

Withanolide A Withanolide B

2.90

Totals

3474330

100.00