SPDS SET 2: ASH-01

QUALITY CONTROL DEPARTMENT

Name of Sample Method Name Data File Name

: Withania somnifera : RD - 1162 : RM

: C:\Methods\WITHANIA.met

: D:\LC10-ATvp\2007\Method validation\Apr 2007\020407\S7-Rep2

Vial No

: 10008

Injection Volume

: 20

Date & Time

: 4/4/07 1:05:44 AM

0.150

0.125

17.217

0.100

0.075

Volts

21.500

23.892

0.050

23.008

0.025

26.958

0.000

22.008

0

5

10

15

20

25

30

35

40

45

Minutes

Detector A - 1 (227nm)

Pk # Retention Time Name

Area Area Percent

1 2 3 4 5 6

17.217 21.500 22.008 23.008 23.892 26.958

Withanoside IV Withanoside V Withaferin A

512636 301908 880299 243613 259210

22.91 13.49 39.35 10.89 11.59

Withastramonolide

Withanolide A Withanolide B

39607

1.77

Totals

2237273

100.00