SPDS SET 2: ASH-01

QUALITY CONTROL DEPARTMENT

Name of Sample Method Name Data File Name

: Withania somnifera : RD - 1162 : RM

: C:\Methods\WITHANIA.met

: D:\LC10-ATvp\2007\Method validation\Apr 2007\020407\S7-Rep1

Vial No

: 10008

Injection Volume

: 20

Date & Time

: 4/4/07 12:19:51 AM

0.150

0.125

17.217

0.100

0.075

Volts

21.508

0.050

23.900

23.008

0.025

26.958

0.000

22.008

0

5

10

15

20

25

30

35

40

45

Minutes

Detector A - 1 (227nm)

Pk # Retention Time Name

Area Area Percent

1 2 3 4 5 6

17.217 21.508 22.008 23.008 23.900 26.958

Withanoside IV Withanoside V Withaferin A

505117 298751 872090 239104 255962

22.86 13.52 39.47 10.82 11.59

Withastramonolide

Withanolide A Withanolide B

38216

1.73

Totals

2209240

100.00