SPDS SET 2: ASH-01

Dilution-3: 5ml of Dilution-2 solution was pipetted to10ml volumetric flask and made up to 10ml with acetonitrile.

Table-7

Sl. No.

Reference standard

Concentrations (mcg/ml)

1.

165.15

Withanoside IV

2.

150.08

Withanoside V

3.

184.39

Withaferin A

4.

177.65

12-Deoxy withastramonolide

5.

172.76

Withanolide A

6.

168.79

Withanolide B

Dilution-4: 5ml of Dilution-3 solution was pipetted to10ml volumetric flask and made up to 10ml with acetonitrile.

Table-8 Sl. No.

Reference standard

Concentrations (mcg/ml)

1.

82.58

Withanoside IV

2.

75.04

Withanoside V

3.

92.19

Withaferin A

4.

88.83

12-Deoxy withastramonolide

5.

86.38

Withanolide A

6.

84.39

Withanolide B

Dilution-5: 5ml of Dilution-4 solution was pipetted to10ml volumetric flask and made up to 10ml

with acetonitrile.

Table-9 Sl. No.

Reference standard

Concentrations (mcg/ml)

1.

41.29

Withanoside IV

2.

37.52

Withanoside V

3.

46.09

Withaferin A

4.

44.41

12-Deoxy withastramonolide

5.

43.19

Withanolide A

6.

42.19

Withanolide B

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3DJH RI