SPDS SET 2: ASH-01

Table-4: Stock solution Sl. No.

Reference standard

Concentrations (mcg/ml)

1.

1321.20

Withanoside IV

2.

1200.60

Withanoside V

3.

1475.10

Withaferin A

4.

1421.20

12-Deoxy withastramonolide

5.

1382.04

Withanolide A

6.

1350.36

Withanolide B

Dilution-1: 5ml of stock solution was pipetted to10ml volumetric flask and made up to 10ml with acetonitrile.

Table-5

Sl. No.

Reference standard

Concentrations (mcg/ml)

1.

660.60

Withanoside IV

2.

600.30

Withanoside V

3.

737.55

Withaferin A

4.

710.60

12-Deoxy withastramonolide

5.

691.02

Withanolide A

6.

675.18

Withanolide B

Dilution-2: 5ml of Dilution-1 solution was pipetted to10ml volumetric flask and made up to 10ml with acetonitrile.

Table-6

Sl. No.

Reference standard

Concentrations (mcg/ml)

7.

330.30

Withanoside IV

8.

300.15

Withanoside V

9.

368.78

Withaferin A

10.

355.30

12-Deoxy withastramonolide

11.

345.51

Withanolide A

12.

337.59

Withanolide B

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3DJH RI