SPDS SET 2: ASH-01

Precision of Retention time:

4.3.10) Observation

Table-30: Retention time of Withanoside IV

Withanoside IV (mcg/ml)

Different Concentrations

Dilution -6 (20.64)

Dilution -5 (41.29)

Dilution -4 (82.58)

Dilution -3 (165.15)

Dilution -2 (330.30)

Dilution -1 (660.60)

S. solution (1321.20)

Replicate – 1

15.466

15.499

15.523

15.539

15.489

15.732

15.513

Replicate – 2

15.481

15.479

15.533

15.539

15.5

15.53

15.516

Replicate – 3

15.474

15.511

15.512

15.504

15.485

15.502

15.517

Replicate – 4

15.467

15.494

15.513

15.534

15.497

15.519

15.513

Replicate – 5

15.483

15.509

15.52

15.502

15.68

15.515

15.511

Mean

15.474

15.498

15.520

15.524

15.530

15.559

15.514

RSD

0.05

0.08

0.05

0.12

0.54

0.62

0.02

Table-31: Retention time of Withanoside IV in sample (B. No. WS/06Lot10) Replicate Conc-1 (753.6 mg/100ml) Conc-2 (1503.2 mg/100ml) Conc-3 (2096.0 mg/100ml) Replicate - 1 15.529 15.383 15.462 Replicate - 2 15.372 15.388 15.390 Mean 15.451 15.386 15.426 RSD 0.51 0.02 0.23

Table-32: Retention time of Withanoside V

Withanoside V (mcg/ml)

Different Concentrations

Dilution -6 (18.76)

Dilution -5 (37.52)

Dilution -4 (75.04)

Dilution -3 (150.08)

Dilution -2 (300.15)

Dilution -1 (600.30)

S. solution (1200.60)

Replicate – 1

19.651

19.657

19.708

19.721

19.676

19.926

19.688

Replicate – 2

19.641

19.664

19.862

19.686

19.689

19.689

19.695

Replicate – 3

19.658

19.67

19.694

19.691

19.648

19.687

19.702

Replicate – 4

19.635

19.668

19.674

19.691

19.697

19.684

19.706

Replicate – 5

19.665

19.664

19.68

19.688

19.877

19.676

19.704

Mean

19.650

19.665

19.724

19.695

19.717

19.732

19.699

RSD

0.06

0.03

0.40

0.07

0.46

0.55

0.04

1$785$/ 5(0(',(6

3DJH RI