SPDS SET 2: ASH-01

Table-33: Retention time of Withanoside V in sample (B. No. WS/06Lot10) Replicate Conc-1 (753.6 mg/100ml) Conc-2 (1503.2 mg/100ml) Conc-3 (2096.0 mg/100ml) Replicate - 1 19.684 19.534 19.630 Replicate - 2 19.532 19.540 19.562 Mean 19.608 19.537 19.596 RSD 0.39 0.02 0.17

Table-34: Retention time of Withaferin A

Withaferin A (mcg/ml)

Different Concentrations

Dilution -6 (23.05)

Dilution -5 (46.09)

Dilution -4 (92.19)

Dilution -3 (184.39)

Dilution -2 (368.78)

Dilution -1 (737.55)

S. solution (1475.10)

Replicate – 1

20.32

20.328

20.374

20.383

20.337

20.563

20.337

Replicate – 2

20.311

20.332

20.351

20.353

20.349

20.347

20.344

Replicate – 3

20.327

20.341

20.362

20.355

20.314

20.344

20.352

Replicate – 4

20.306

20.339

20.345

20.357

20.361

20.342

20.352

Replicate – 5

20.333

20.336

20.349

20.355

20.525

20.335

20.352

Mean

20.319

20.335

20.356

20.361

20.377

20.386

20.347

RSD

0.05

0.03

0.06

0.06

0.41

0.49

0.03

Table-35: Retention time of Withaferin A in sample (B. No. WS/06Lot10) Replicate Conc-1 (753.6 mg/100ml) Conc-2 (1503.2 mg/100ml) Conc-3 (2096.0 mg/100ml) Replicate - 1 20.362 20.224 20.308 Replicate - 2 20.223 20.227 20.248 Mean 20.293 20.226 20.278 RSD 0.48 0.01 0.15

Table-36: Retention time of 12-Deoxy Withastramonolide

12-Deoxy withastramonolide (mcg/ml)

Different Concentrations

Dilution -6 (22.21)

Dilution -5 (44.41)

Dilution -4 (88.83)

Dilution -3 (177.65)

Dilution -2 (355.30)

Dilution -1 (710.60)

S. solution (1421.20)

Replicate – 1

21.351

21.351

21.391

21.394

21.36

21.542

21.36

Replicate – 2

21.341

21.358

21.367

21.367

21.366

21.366

21.365

Replicate – 3

21.357

21.361

21.38

21.376

21.341

21.363

21.373

Replicate – 4

21.338

21.362

21.365

21.367

21.382

21.362

21.37

Replicate – 5

21.356

21.358

21.37

21.376

21.51

21.353

21.372

Mean

21.349

21.358

21.375

21.376

21.392

21.397

21.368

RSD

0.04

0.02

0.05

0.05

0.32

0.38

0.03

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