SPDS SET 2: ASH-01

The mean response factor for Withanoside V between the concentration 18.76 to 300.15 mcg/ml is

7781.07.

Table-69:

Withanoside V (mcg/ml)

Different Concentrations

Dilution -6 (18.76)

Dilution -5 (37.52)

Dilution -4 (75.04)

Dilution -3 (150.08)

Dilution -2 (300.15)

Dilution -1 (600.30)

S. solution (1200.60)

Mean

151167

297773

585013

1147767

2241004

4335433

8721109

7222.11

7263.96

Response factor Area / concentration Mean (Dilution 2 to 6)

8058.19

7936.65

7796.28

7647.96

7466.28

7781.072

±5.0% of 7781 is7391 to 8170

The mean response factor for Withaferin A between the concentration 23.05 to 184.39 mcg/ml is 24274.

Table-70:

Withaferin A (mcg/ml)

Different Concentrations

Dilution -6 (23.05)

Dilution -5 (46.09)

Dilution -4 (92.19)

Dilution -3 (184.39)

Dilution -2 (368.78)

Dilution -1 (737.55)

S. solution (1475.10)

Mean

573348

1134003

2224593

4331341

8326374

14752690

21454279

22578.47

20002.29

14544.29

Response factor Area / concentration Mean (Dilution 3 to 6)

24875.80

24600.44

24129.54

23490.43

24274.053

±5.0% of 24274 is 23060 to 25487

The mean response factor for 12-Deoxy withastramonolide between the concentration 22.21 to 177.65 mcg/ml is 27917.

Table-71:

12-Deoxy withastramonolide (mcg/ml)

Different Concentrations

Dilution -6 (22.21)

Dilution -5 (44.41)

Dilution -4 (88.83)

Dilution -3 (177.65)

Dilution -2 (355.30)

Dilution -1 (710.60)

S. solution (1421.20)

Mean

634878

1255825

2466826

4802093

9146389

14844246

18055804

25742.72

20889.74

12704.62

Response factor Area / concentration

28590.08

28276.39

27771.75

27031.20

Mean (Dilution 3 to 6)

27917.36

±5.0% of 27917 is 26521 to 29312

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