SPDS SET 2: ASH-01

The mean response factor for Withanolide A between the concentration 21.59 to 172.76 mcg/ml is

24803.79.

Table-72:

Withanolide A (mcg/ml)

Different Concentrations

Dilution -6 (21.59)

Dilution -5 (43.19)

Dilution -4 (86.38)

Dilution -3 (172.76)

Dilution -2 (345.51)

Dilution -1 (691.02)

S. solution (1382.04)

Mean

551256

1084444

2127011

4138065

7961571

13486375

13768071

23042.95

19516.62

9962.14

Response factor Area / concentration Mean (Dilution 3 to 6)

25527.76

25109.41

24624.59

23953.38

24803.79

±5.0% of 24803 is 23562 to 26043

The mean response factor for Withanolide B between the concentration 21.09 to 168.79 mcg/ml is

25634.15.

Table-73:

Withanolide B (mcg/ml)

Different Concentrations

Dilution -6 (21.09)

Dilution -5 (42.19)

Dilution -4 (84.39)

Dilution -3 (168.79)

Dilution -2 (337.59)

Dilution -1 (675.18)

S. solution (1350.36)

Mean

552858

1094892

2153855

4197521

7949510

12726145

12163116

23547.83

18848.52

9007.31

Response factor Area / concentration Mean (Dilution 3 to 6)

26202.60

25946.08

25520.36

24867.57

25634.15

±5.0% of 25634 is 24352 to 26915

Though the linearity is observed through out the six concentrations ranging from 20 to 600mcg. The following limits are considered as a better working range based on linearity and closeness

(within ± 5%) to mean response factor.

Table-74:

Name of the analyte

Concentration (mcg/ml)

Withanoside IV

20.64 to 330.3

Withanoside V

18.76 to 300.15

Withaferin A

23.05 to 184.39

12-Deoxy withastramonolide

22.21 to 177.65

Withanolide A

21.59 to 172.76

Withanolide B

21.09 to 168.79

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