SPDS SET 2: ASH-01

NATURAL REMEDIES PVT LIMITED QUALITY CONTROL DEPARTMENT

Acquired by Sample Name

: Admin

: Withania : std mix

Sample ID

: Dilution : I

Vail #

: 12

Injection Volume Data File Name Method File Name Batch File Name Report File Name Data Acquired Data Processed

: 20 uL

: Lin029.lcd

: Withaina.met.lcm : Batch 01.lcb : Calibration.lcr

: 1/14/2007 10:06:23 PM : 1/23/2007 2:32:07 AM

D:\LC2010AHT\2007\Method validation\Withania somnifera\Jan 2007\Lin029.lcd

mV

1Det.A Ch1

4

5

2000

6

3

1500

1000

1

2

500

0

0

5

10

15

20

25

30

35

40

45

min

Det.A Ch1/227nm 1

Detector A Ch1 227nm Peak # Ret. Time

Area 4815725 4338646 14788044 14874117 13502916 12723957 65043405

Height

Area %

Name

1 2 3 4 5 6 Total

15.519 19.684 20.342 21.362 22.223 25.521

770932 612656 2008178 2176641 2064485 2038641 9671533

7.404 6.670

Withanoside IV Withanoside V Withaferin A 12- Deoxy withastramonolide

22.736 22.868 20.760 19.562 100.000

Withanollide A Withanolide B

D:\LC2010AHT\2007\Method validation\Withania somnifera\Jan 2007\Lin029.lcd