SPDS SET 2: ASH-01

NATURAL REMEDIES PVT LIMITED QUALITY CONTROL DEPARTMENT

Acquired by Sample Name

: Admin

: Withania : std mix

Sample ID

: Dilution : I

Vail #

: 12

Injection Volume Data File Name Method File Name Batch File Name Report File Name Data Acquired Data Processed

: 20 uL

: Lin030.lcd

: Withaina.met.lcm : Batch 01.lcb : Calibration.lcr

: 1/14/2007 10:52:01 PM : 1/23/2007 2:31:16 AM

D:\LC2010AHT\2007\Method validation\Withania somnifera\Jan 2007\Lin030.lcd

mV

1Det.A Ch1

4

5

2000

6

3

1500

1000

1

2

500

0

0

5

10

15

20

25

30

35

40

45

min

Det.A Ch1/227nm 1

Detector A Ch1 227nm Peak # Ret. Time

Area 4818317 4345473 14782253 14859008 13491381 12720153 65016585

Height

Area %

Name

1 2 3 4 5 6 Total

15.515 19.676 20.335 21.353 22.213 25.513

768546 613872 2013446 2175533 2064396 2034360 9670152

7.411 6.684

Withanoside IV Withanoside V Withaferin A 12- Deoxy withastramonolide

22.736 22.854 20.751 19.564 100.000

Withanollide A Withanolide B

D:\LC2010AHT\2007\Method validation\Withania somnifera\Jan 2007\Lin030.lcd