SPDS SET 2: ASH-01

NATURAL REMEDIES PVT LIMITED QUALITY CONTROL DEPARTMENT

Acquired by Sample Name

: Admin

: Withania : std mix

Sample ID

: Dilution : III

Vail #

: 10

Injection Volume Data File Name Method File Name Batch File Name Report File Name Data Acquired Data Processed

: 20 uL

: Lin017.lcd

: Withaina.met.lcm : Batch 01.lcb : Calibration.lcr

: 1/14/2007 12:48:57 PM : 1/23/2007 2:08:43 AM

D:\LC2010AHT\2007\Method validation\Withania somnifera\Jan 2007\Lin017.lcd

mV

1Det.A Ch1

4

750

6

5

3

500

250

1

2

0

0

5

10

15

20

25

30

35

40

45

min

Det.A Ch1/227nm 1

Detector A Ch1 227nm Peak # Ret. Time

Area 1278440 1146843 4337796 4809678 4140860 4205282 19918898

Height

Area %

Name

1 2 3 4 5 6 Total

15.538 19.686 20.353 21.367 22.230 25.521

203561 163686 638520 802935 687017 727155 3222873

6.418 5.758

Withanoside IV Withanoside V Withaferin A 12- Deoxy withastramonolide

21.777 24.146 20.789 21.112 100.000

Withanollide A Withanolide B

D:\LC2010AHT\2007\Method validation\Withania somnifera\Jan 2007\Lin017.lcd