SPDS SET 2: ASH-01

NATURAL REMEDIES PVT LIMITED QUALITY CONTROL DEPARTMENT

Acquired by Sample Name

: Admin

: Withania : std mix

Sample ID

: Dilution : III

Vail #

: 10

Injection Volume Data File Name Method File Name Batch File Name Report File Name Data Acquired Data Processed

: 20 uL

: Lin018.lcd

: Withaina.met.lcm : Batch 01.lcb : Calibration.lcr

: 1/14/2007 1:34:44 PM : 1/23/2007 2:10:33 AM

D:\LC2010AHT\2007\Method validation\Withania somnifera\Jan 2007\Lin018.lcd

mV

1Det.A Ch1

4

750

6

5

3

500

250

1

2

0

0

5

10

15

20

25

30

35

40

45

min

Det.A Ch1/227nm 1

Detector A Ch1 227nm Peak # Ret. Time

Area 1269142 1141122 4310236 4778467 4118314 4174239 19791519

Height

Area %

Name

1 2 3 4 5 6 Total

15.504 19.691 20.355 21.376 22.241 25.536

201799 162126 630249 796159 678874 722038 3191245

6.413 5.766

Withanoside IV Withanoside V Withaferin A 12- Deoxy withastramonolide

21.778 24.144 20.808 21.091 100.000

Withanollide A Withanolide B

D:\LC2010AHT\2007\Method validation\Withania somnifera\Jan 2007\Lin018.lcd