SPDS SET 2: ASH-01

NATURAL REMEDIES PVT LIMITED QUALITY CONTROL DEPARTMENT

Acquired by Sample Name

: Admin

: Withania : std mix

Sample ID

: Dilution : III

Vail #

: 10

Injection Volume Data File Name Method File Name Batch File Name Report File Name Data Acquired Data Processed

: 20 uL

: Lin019.lcd

: Withaina.met.lcm : Batch 01.lcb : Calibration.lcr

: 1/14/2007 2:20:29 PM : 1/23/2007 2:12:10 AM

D:\LC2010AHT\2007\Method validation\Withania somnifera\Jan 2007\Lin019.lcd

mV

1Det.A Ch1

4

750

6

5

3

500

250

1

2

0

0

5

10

15

20

25

30

35

40

45

min

Det.A Ch1/227nm 1

Detector A Ch1 227nm Peak # Ret. Time

Area 1278566 1152990 4335168 4808531 4144205 4205370 19924828

Height

Area %

Name

1 2 3 4 5 6 Total

15.534 19.691 20.357 21.367 22.227 25.514

203336 163525 639586 802957 687096 725686 3222187

6.417 5.787

Withanoside IV Withanoside V Withaferin A 12- Deoxy withastramonolide

21.758 24.133 20.799 21.106 100.000

Withanollide A Withanolide B

D:\LC2010AHT\2007\Method validation\Withania somnifera\Jan 2007\Lin019.lcd