SPDS SET 2: ASH-01

NATURAL REMEDIES PVT LIMITED QUALITY CONTROL DEPARTMENT

Acquired by Sample Name

: Admin

: Withania : std mix

Sample ID

: Dilution : VI

Vail #

: 7

Injection Volume Data File Name Method File Name Batch File Name Report File Name Data Acquired Data Processed

: 20 uL

: Lin002.lcd

: Withaina.met.lcm : Batch 01.lcb : Calibration.lcr

: 1/14/2007 1:22:36 AM : 1/23/2007 1:48:07 AM

D:\LC2010AHT\2007\Method validation\Withania somnifera\Jan 2007\Lin002.lcd

mV

1Det.A Ch1

750

500

250

4

6

5

3

1

2

0

0

5

10

15

20

25

30

35

40

45

min

Det.A Ch1/227nm 1

Detector A Ch1 227nm Peak # Ret. Time

Area

Height

Area %

Name

1 2 3 4 5 6 Total

15.481 19.641 20.311 21.341 22.209 25.511

166038 151121 573224 634565 550821 552800 2628569

26677 21442 84096 105947 90976 96186 425324

6.317 5.749

Withanoside IV Withanoside V Withaferin A 12- Deoxy withastramonolide

21.807 24.141 20.955 21.030 100.000

Withanollide A Withanolide B

D:\LC2010AHT\2007\Method validation\Withania somnifera\Jan 2007\Lin002.lcd