SPDS SET 2: ASH-01

NATURAL REMEDIES PVT LIMITED QUALITY CONTROL DEPARTMENT

Acquired by Sample Name

: Admin

: Withania somnifera : WS / 06 lot 10

Sample ID

: 1500 mg / 100 ml : Trl 1

Vail #

: 47

Injection Volume Data File Name Method File Name Batch File Name Report File Name Data Acquired Data Processed

: 20 uL

: Rep-RP-II005.lcd : Withaina.met.lcm : Batch 01.lcb : Calibration.lcr

: 1/16/2007 4:50:36 PM : 1/23/2007 3:02:09 AM

D:\LC2010AHT\2007\Method validation\Withania somnifera\Jan 2007\Rep-RP-II005.lcd

mV

1Det.A Ch1

400

3

300

1

200

4

5

2

100

6

0

0

5

10

15

20

25

30

35

40

45

min

Det.A Ch1/227nm 1

Detector A Ch1 227nm Peak # Ret. Time

Area

Height

Area %

Name

1 2 3 4 5 6 Total

15.388 19.540 20.227 21.262 22.128 25.450

949957 793298 2173830 1088292 797140 242191 6044708

167823 114653 304832 177034 139023 43112 946478

15.716 13.124 35.963 18.004 13.187 4.007 100.000

Withanoside IV Withanoside V Withaferin A 12- Deoxy withastramonolide

Withanollide A Withanolide B

D:\LC2010AHT\2007\Method validation\Withania somnifera\Jan 2007\Rep-RP-II005.lcd