SPDS SET 2: ASH-01

NATURAL REMEDIES PVT LIMITED QUALITY CONTROL DEPARTMENT

Acquired by Sample Name

: Admin

: Withania somnifera : WS / 06 lot 10

Sample ID

: 1500 mg / 100 ml : Trl 2

Vail #

: 48

Injection Volume Data File Name Method File Name Batch File Name Report File Name Data Acquired Data Processed

: 20 uL

: Rep-RP-II006.lcd : Withaina.met.lcm : Batch 01.lcb : Calibration.lcr

: 1/16/2007 5:36:20 PM : 1/23/2007 3:03:19 AM

D:\LC2010AHT\2007\Method validation\Withania somnifera\Jan 2007\Rep-RP-II006.lcd

mV

400

1Det.A Ch1

3

300

1

200

4

5

2

100

6

0

0

5

10

15

20

25

30

35

40

45

min

Det.A Ch1/227nm 1

Detector A Ch1 227nm Peak # Ret. Time

Area

Height

Area %

Name

1 2 3 4 5 6 Total

15.383 19.534 20.224 21.261 22.126 25.449

949797 783540 2152710 1075418 784022 239205 5984692

166616 112934 300796 174559 137155 42555 934615

15.870 13.092 35.970 17.969 13.100 3.997 100.000

Withanoside IV Withanoside V Withaferin A 12- Deoxy withastramonolide

Withanollide A Withanolide B

D:\LC2010AHT\2007\Method validation\Withania somnifera\Jan 2007\Rep-RP-II006.lcd