SPDS SET 2: ASH-01

NATURAL REMEDIES PVT LIMITED QUALITY CONTROL DEPARTMENT

Acquired by Sample Name

: Admin

: Withania somnifera : WS / 06 lot 10

Sample ID

: 1000 mg / 100 ml : Trl 2

Vail #

: 33

Injection Volume Data File Name Method File Name Batch File Name Report File Name Data Acquired Data Processed

: 20 uL

: Rep-RP004.lcd : Withaina.met.lcm : Batch 01.lcb : Calibration.lcr

: 1/16/2007 3:50:42 AM : 1/23/2007 3:11:41 AM

D:\LC2010AHT\2007\Method validation\Withania somnifera\Jan 2007\Rep-RP004.lcd

mV

1Det.A Ch1

250

200

3

150

1

4

100

5

2

50

6

0

0

5

10

15

20

25

30

35

40

45

min

Det.A Ch1/227nm 1

Detector A Ch1 227nm Peak # Ret. Time

Area

Height

Area %

Name

1 2 3 4 5 6 Total

15.528 19.694 20.369 21.377 22.236 25.525

597742 503383 1365742 683044 481718 152164 3783792

105658 72291 192602 112832 86757 27293 597433

15.797 13.304 36.095 18.052 12.731 4.021 100.000

Withanoside IV Withanoside V Withaferin A 12- Deoxy withastramonolide

Withanollide A Withanolide B

D:\LC2010AHT\2007\Method validation\Withania somnifera\Jan 2007\Rep-RP004.lcd