SPDS SET 2: ASH-01

NATURAL REMEDIES PVT LIMITED QUALITY CONTROL DEPARTMENT

Acquired by Sample Name

: Admin

: Withania somnifera : WS / 06 lot 10

Sample ID

: 1500 mg / 100 ml : Trl 1

Vail #

: 34

Injection Volume Data File Name Method File Name Batch File Name Report File Name Data Acquired Data Processed

: 20 uL

: Rep-RP005.lcd : Withaina.met.lcm : Batch 01.lcb : Calibration.lcr

: 1/16/2007 4:36:25 AM : 1/23/2007 3:13:11 AM

D:\LC2010AHT\2007\Method validation\Withania somnifera\Jan 2007\Rep-RP005.lcd

mV

1Det.A Ch1

400

3

300

1

4

200

5

2

100

6

0

0

5

10

15

20

25

30

35

40

45

min

Det.A Ch1/227nm 1

Detector A Ch1 227nm Peak # Ret. Time

Area 1042497 869310 2368568 1186938 851674 266074 6585062

Height

Area %

Name

1 2 3 4 5 6 Total

15.513 19.683 20.358 21.372 22.236 25.535

184367 124386 332886 195375 151330 47554 1035898

15.831 13.201 35.969 18.025 12.933 4.041 100.000

Withanoside IV Withanoside V Withaferin A 12- Deoxy withastramonolide

Withanollide A Withanolide B

D:\LC2010AHT\2007\Method validation\Withania somnifera\Jan 2007\Rep-RP005.lcd