SPIFAN Nutrients ERP Book_9-29-15

Chlor-03 SLV FOR ERP USE ONLY DO NOT DISTRIBUTE

Step2: Make up the volume with DI water up to 20 mL. Add g of DI water and record the total weight. Cap the bottle and shake until well dissolved. Reconstituted powder samples must be used immediately. Shake for 2-3 min.

Step3: Transfer 12 mL of it to a 50 mL Amicon Ultra-15 Centrifugal filter device and cap. Centrifuge for 60 min at 5000 rpm at 20 o C.

Step4. Filter through 0.2µm filter before analysis.

2. Ready to Feed Formula

Step1: Transfer 12 mL of RTF to a 50 mL Amicon Ultra-15 Centrifugal filter device and cap. Centrifuge for 60 min at 5000 rpm at 20 o C.

Step2: Filter through 0.2µm filter before analysis.

3. Adult Nutritional High fat and High Protein formula*

Step1: Place a 20 mL polypropylene bottle on the balance and tare it. Add 5 g of liquid and record the weight.

Step2: Make up the volume with DI water up to 20 mL Add g of DI water and record the total weight. Cap the bottle and shake until well dissolved. Shake for 2-3 min.

Step3: Transfer the 12 mL of it to a 50 mL Amicon Ultra-15 Centrifugal filter device and cap. Centrifuge for 60 min at 5000rpm at 20 o C.

Step4: Filter through 0.2µm filter before analysis.

* Due to the high viscosity of adult nutritional high fat and high Protein formula, these samples (both placebos and fortified samples) were diluted 4 times before centrifugation.

Validation protocol

SLV guidelines.—SPIFAN SLV recommended guidelines were followed.

System suitability Verify check standards daily at the lowest point and midpoint of the analytical range throughout the batch run. Recalibrate the system when the percent error of the check standards is >5%. Linearity/Calibration Fit : Perform three separate experiments using independently prepared standards at seven concentration levels that span the desired working range. In this study, the relative error of back-calculated concentrations within the desired working range was <5%.

Precision studies - Analyze samples selected for precision studies on each of six days using multiple instruments. Prepare fresh reagents each day.