SPIFAN Nutrients ERP Book_9-29-15

FOS-04 FOR ERP USE ONLY DO NOT DISTRIBUTE

Rules for determining fructan type 1.

DP 3.6-4 (CF = 1.233, #1 in Table 2-3 in Part 2) - Presence of GF 3

and GF 4

with the absence of peaks after ~36

minutes.

2. DP 5.49-10 (CF = 1.0679, #2 in Table 2-3 in Part 2) - Presence of both GF 3

and GF 4

, along with peaks after ~36

minutes.

3. DP 11-50 (CF= 0.9526, #3 in Table 2-3 in Part 2) - Absence of GF 3

and GF 4

and presence of peaks after ~ 36

minutes.

Additional Observations 1.

Absence of significant peaks after ~5 minutes means scFOS (DP 3.6). See Figure 1-2 for an example. 2. Oligofructose containing materials derived from the partial hydrolysis of chicory inulin often display a characteristic peak with significant tailing at ~ 5 minutes. See Figure 1-3 for an example. 3. Presence of significant peaks after ~36 minutes means it cannot be scFOS or oligofructose. Contains some higher DP inulin type material. See Figure 1-4 for an example.

Example determinations 1.

Figures 1-5a and 1-5b show an example of product #5 (SPIFAN matrix Placebo Child Formula Powder). As shown in Figure 1-5b there are no significant peaks after 36 minutes as is shown in Synergy 1 or HP therefore it must belong to the lowest average DP range. 2. Figure 1-6 shows powdered milk based adult powder containing Synergy 1. This can be seen by the presence of both GF 3 /GF 4 as well as peaks after 36 minutes. 3. Figure 1-7 shows a powdered infant formula containing GOS that was spiked with HP. This can be confirmed by the absence of GF 3 /GF 4 as well as peaks after 36 minutes.

Figure 1-2 scFOS STD

Figure 1-3 Synergy 1 STD

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