SPIFAN Nutrients ERP Book_9-29-15

GOS-02 SLV FOR ERP USE ONLY DO NOT DISTRIBUTE

Single Lab Validation Report for GOS in Infant Formula and Adult Nutritionals

Linear Calibration Curve Lactose

1.4

1.2

y = 0.0696x + 0.0003 R² = 0.9994

1

0.8

0.6

0.4

Ratio Area/Area IS

0.2

0

0.00

5.00

10.00

15.00

20.00

Lactose Concentration (µg/mL)

Figure 2: Linear calibration curve for lactose

Although the R 2 values were acceptable, the residue plots of the back-calculated concentrations showed parabolic trending on the linear fit (Figure 3 and 4).

Residu Plot Galactose Linear

25

20

15

10

5

0

0.00

5.00

10.00

15.00

20.00

-5

% deviation from actual concentration

-10

Galactose Concentration (µg/mL)

Figure 3: Residue plot of the deviation from the actual concentration by linear calibration for galactose

7