SPIFAN Nutrients ERP Book_9-29-15

GOS-02 SLV FOR ERP USE ONLY DO NOT DISTRIBUTE

Single Lab Validation Report for GOS in Infant Formula and Adult Nutritionals

Quadratic Calibration Curve Lactose

1.4

1.2

y = -0.0001x 2 + 0.0716x - 0.0038 R² = 0.9995

1

0.8

0.6

0.4

Ratio Area/Area IS

0.2

0

0.00

5.00

10.00

15.00

20.00

Lactose Concentration (µg/mL)

Figure 6: Quadratic calibration curve for lactose

The residue plots of the back-calculated concentrations on the quadratic fit showed a deviation of less than 1.5% for the galactose (Figure 7) and of less than 8% for the lactose (Figure 8) without parabolic trending. Therefore, calculations for precision and accuracy are based on quadratic fit.

Residue Plot Galactose Quadratic

1

0.5

0

0.00

5.00

10.00

15.00

20.00

-0.5

-1

% deviation from actual concentration

-1.5

Galactose Concentration (µg/mL)

Figure 7: Residue plot of the deviation from the actual concentration by quadratic calibration for galactose

9