SPIFAN Nutrients ERP Book_9-29-15

GOS-02 SLV FOR ERP USE ONLY DO NOT DISTRIBUTE

Single Lab Validation Report for GOS in Infant Formula and Adult Nutritionals

5.2 Raw data – Method Precision Date*

Instrument**

Result A

Result B

8 IF Powder Partially Hydrolyzed Milk Based (1172572116) 20.01.2015 ICS3000

0.31 0.29 0.30 0.32 0.32 0.31 0.24 0.24 0.22 0.23 0.22 0.25 0.19 0.22 0.19 0.16 0.17 0.20

0.30 0.30 0.30 0.33 0.31 0.32 0.25 0.24 0.24 0.23 0.23 0.24 0.19 0.22 0.18 0.17 0.18 0.20

21.01.2015 22.01.2015 27.01.2015 28.01.2015 29.01.2015

ICS5000 ICS3000 ICS5000 ICS3000 ICS5000

13 Infant Formula Powder Milk Based (D04HTCVV) 20.01.2015 ICS3000

21.01.2015 22.01.2015 27.01.2015 28.01.2015 29.01.2015

ICS5000 ICS3000 ICS5000 ICS3000 ICS5000

15 Infant Formula RTF Milk Based (SPIFAN Control Milk Form) 01.06.2015 ICS3000

02.06.2015 03.06.2015 04.06.2015 05.06.2015 08.06.2015

ICS5000 ICS3000 ICS5000 ICS3000 ICS5000

* Date = date of preparative separation ** Instrument = instrument used for quantification

5.3 Raw data – Method accuracy The samples were freshly prepared for each spiking experiment. The spiking was performed by the addition of 1% GOS solution to the product so that the GOS concentration in the product would be 0.2, 1.0, 2.5 and 5.0 g/100 g. Since the samples were not reconstituted prior to analyses, the actual GOS concentration calculated on reconstitution were 0.02, 0.1, 0.3 and 0.6 g/100 g (i.e. a factor 9 lower).

21