SPIFAN Nutrients ERP Book_9-29-15

Carn-06 - Validation Report FOR ERP USE ONLY DO NOT DISTRIBUTE

Appendix 1: Linearity

Carnitine:

Actual Concentration (ng/g)

IS Actual Concentration (ng/g)

Area (counts)

IS Area (counts)

Concentration ratio

Area ratio

std 1 std 2 std 3 std 4 std 5 std 6 std 7 std 8 std 9 std 1 std 2 std 3 std 4 std 5 std 6 std 7 std 8 std 9

4,6129 9,0881 38,1799 74,0862 146,2597 256,4854 550,7591 1000,0994 1550,7960 4,6129 9,0881 38,1799 74,0862 146,2597 256,4854 550,7591 1000,0994 1550,7960

217,8116 225,4382 209,5343 221,8754 223,4094 218,8944 222,5433 221,0921 221,9617 217,8116 225,4382 209,5343 221,8754 223,4094 218,8944 222,5433 221,0921 221,9617

3329,5 7139,4

160220,7 181885,2

0,021178395 0,020780686 0,040313044 0,039252491 0,182213127 0,175655523 0,333909032 0,326066704 1,171731209 1,13343267 2,474840177 2,309526344 4,523451539 4,337842857 6,986772943 6,674058219 0,021178395 0,022730696 0,040313044 0,038167521 0,182213127 0,178401211 0,333909032 0,324567286 1,171731209 1,144839091 2,474840177 2,367995416 4,523451539 4,433062372 6,986772943 6,715054693 0,6546712 0,661242287 0,6546712 0,633634036

29263,0 166593,2 55518,4 170267,0 112370,8 169939,0 183940,7 162286,4 363396,5 157346,8 611060,3 140867,3 807552,3 120998,7 30022,4 168285,8 55438,6 170807,8 111210,2 175511,7 188626,3 164762,3 354499,4 149704,4 612058,0 138066,6 892823,7 132958,5 3769,3 6802,4 165826,3 178225,4

SUMMARY OUTPUT

Regression Statistics

Multiple R R Square

0,999913128

0,999826263 Adjusted R Square 0,999815404 Standard Error 0,030825052 Observations 18

ANAVA

df Significance F Regression 1 87,49023889 87,49023889 92077,17128 1,63032E-31 Residual 16 0,015202941 0,000950184 Total 17 87,50544183 SS MS F

Coefficients

Std. Error

t Stat

P-value

Lower 95%

Upper 95%

Intercept 0,00455914 0,009271008 0,491763144 0,629565074 -0,01509451 0,024212798 Concentration ratio 0,959609823 0,003162414 303,4422042 1,63032E-31 0,95290580 0,966313841

RESIDUAL OUTPUT Observation Predicted Area

Residuals

1 2 3 4 5 6 7 8

0,024882135 -0,004101449 0,043243933 -0,003991442 0,179412646 -0,003757124 0,324981527 0,001085177 0,632788054 0,028454233 1,128963918 0,004468752 2,379440084 -0,069913739 4,345307669 -0,007464813