SPIFAN Nutrients ERP Book_9-29-15

BVit-02 - MEF FORM FOR ERP USE ONLY DO NOT DISTRIBUTE

Table 5. Calibration standards and LOQ for

Total B1 Total B2 Total B3 Total B6

Thiamine Riboflavin Nicotinamide Nicotinic acid Pyridoxal

Pyridoxamine Pyridoxine

Vitamin

ng/mL

ng/mL

ng/mL

ng/mL

ng/mL

ng/mL

ng/mL

ng/mL

ng/mL

ng/mL

ng/mL

WS1 WS2 WS3 WS4 WS5 WS6

0.196 0.489 0.978 1.956 4.890 9.780

0.173 0.434 0.867 1.734

1.903 4.758 9.515 19.03

0.255 0.638 1.276 2.551 6.378

0.196 0.489 0.978 1.956 4.890 9.780

0.173 0.434 0.867 1.734 4.335 8.671

1.766 4.416 8.831

0.137 0.342 0.684 1.368 3.420 6.840

0.014 0.034 0.069 0.137 0.343 0.685

0.016 0.041 0.082 0.164 0.410 0.820

0.225 0.563 1.125 2.250 5.625

17.662 44.156 88.312

4.335 47.576

8.671 95.152 12.755

11.250

LOQ*

9.79 ≤ 20

8.65 ≤ 30

95.2

12.8 ug/100g ≤ 10 ug/100g

SMPR LOQ

≤ 200

* LOQ is calculated by multiplying the lowest calibration standard by the dilution factor (500) and converting for units