SPIFAN Nutrients ERP Book_9-29-15

Carn-06 (Choline Validation Report) FOR ERP USE ONLY DO NOT DISTRIBUTE

t / min 30 60 90 120 150 180 240 300 360 From the data (see appendix 4) it is seen that a saponification time of 240 min is sufficient to convert all bound choline to the free forms for all the tested matrixes. Choline: 0 (free)

Saponification 100°C

1000 1500 2000 2500 3000

raw egg

Petfood

Infant formula

0 500

Choline (mg/kg)

Cornflakes

Fishfeed

Time (min)