AOAC 133rd Annual Meeting - Final Program

Scientific Sessions | Tuesday

3:00 PM Global Initiatives in the Development of Mycotoxin Reference Materials Melissa Phillips, U.S. National Institute of Standards and Technology, Hongmei Li, National Institute of Metrology, P.R. China, Eliane C. P. do Rego, INMETRO – National Institute of Metrology, Quality and Technology, Brazil, Maria Fernandes- Whaley, National Metrolgoy Institute of South Africa, Jeremy E. Melanson, National Research Council Canada, Ralf D. Josephs, Gustavo Martos, Bureau International des Poids et Mesures (BIPM), France Reference materials (RMs) for calibration and validation are critical to the quality assurance of measurements made in foods and dietary supplements to adequately represent challenges in extraction, digestion, separation, and/or detection of analytes of interest. To this end, National Metrology Institutes (NMIs) around the world are working to develop new and improved tools for the measurement of mycotoxins. For example, the Bureau International des Poids et Mesures has developed a Capacity Building and Knowledge Transfer program for strengthening mycotoxin metrology infrastructure among NMIs which has focused initially on the development of calibration materials. For ochratoxin A, INMETRO Brazil has developed a coffee matrix CRM and the National Research Council Canada has devel- oped labeled and unlabeled calibration CRMs as well as a rye flour matrix CRM. The National Metrology Institute of South Africa has an active proficiency testing program for aflatoxins in peanuts, and NIST has recently released an incurred CRM for multiple mycotoxins in corn. This presentation will highlight these examples of global initiatives in the development of reference materials to support the accurate determination of mycotoxins in foods. 3:20 PM Certified Reference Materials and Metrological Traceability Assurance for Mycotoxin Analysis Hongmei Li, National Institute of Metrology With the signing of the joint declaration among BIPM-OIML- ILAC-ISO and issue of new international standards, ISO/ IEC17025 and ISO13528, the traceability of metrology has become increasingly important in mutual recognition of measure- ments in various fields. Through the evaluation of measurement capability of different testing laboratories in interlaboratory comparisons, it is found that metrological traceability of cali- brants becomes a critical factor affecting the test results. Reference materials from different sources with incongruous qualities may result in different values. Establishing metrological traceability is a prerequisite to obtaining metrological compa- rability of measurements. This presentation summarizes the commonly used mycotoxin analytical methods and current status of mycotoxin reference materials in order to find the key factors that affect the measurement results of mycotoxin. And chemical measurement system is described and the Capability Building and Knowledge Transfer Programme under the leadership of International Bureau of Weights and Measures is introduced as

an example to show the experience of establishment of myco- toxin measurement system. The aim of this presentation is to give advice to stakeholders in the field of chemical measurement that great effort is needed to promote the effective use of global certified reference materials resources, strengthen metrology infrastructure establishment, and realize “one measurement, global recognition.” 3:35 PM Analytical Method of Multi-Mycotoxins in Spices for Total Diet Study Using Liquid Chromatography Tandem Mass Spectrometry Hyun-Mee Park, Bogyoung Choi, Korea Institute of Science and Technology, Youngsun Lee, Ki Soo Lee, Jung Hee Kim, Korean Institute of Science and Technology Mycotoxins are natural toxins defined as secondary metabo- lites produced by fungi species of Aspergillus, Fusarium , and Penicillium . The objective of this study was to develop and validate analytical methods for multi-mycotoxin (aflatoxins, fumonisins, ochratoxin A, and zearalenone) determination in spices for total diet study. Samples were fortified with 13C isotope labeled mycotoxins as internal standard and extracted with phosphate buffer solution and methanol. Clean-up of the extract was performed by a multi-mycotoxin immunoaffinity column (Myco6in1 ® ), and then analyzed by liquid chroma- tography/tandem mass spectrometry (LC-MS/MS). Method validation was conducted using red pepper as the representative matrix of spice. Standard curves had a good linearity (R2 > 0.99) in all mycotoxin tested. The method detection limit (MDLs) and method quantification limit (MQLs) for eight mycotoxins ranged from 0.005 to 0.3 µg/kg and from 0.015 to 0.9 µg/kg, respectively. The recoveries of all mycotoxins ranged from 89.4 to 117.8% at three concentrations. Intra- and inter-day precisions were in the range of 1.1-10.6% and 1.9-12.3%, respectively. These results are in the acceptable range of Codex Alimentarius Commission (CAC), indicating that these methods are suitable for all mycotoxin determination in several spice samples. 3:55 PM Regulatory Analysis of Mycotoxins in India Kaushik Banerjee, National Research Centre for Grapes Mycotoxins are gaining global importance in the field of food safety due to their deleterious effects on human and animal health. Considering this, the FSSAI has set stringent regulatory maximum levels for various mycotoxin-commodity combina- tions. To facilitate analysis and proficiency testing of mycotoxins within the nation, the FSSAI has recognized ICAR-NRCG as the National Reference Laboratory (NRL) to serve as the nodal agency of the national residue control system on fresh and processed food commodities. The presentation will highlight how the NRL is extensively contributing to developing fit-for-pur- pose methods, and also disseminating those to the stakeholders by organizing various capacity building programs. This talk will briefly discuss the current capacity of Indian food testing laboratories for rapid screening of samples for mycotoxins. It

18 SEPTEMBER 6–12, 2019 SHERATON DENVER DOWNTOWN HOTEL