Review Team (February 2016)

BVit-03 (February 2016) FOR ERP USE ONLY DO NOT DISTRIBUTE

AOAC- SPIFAN-2012-VITAMINS

Calibration Curve

Validation Paramete

Test Group Name

B-complex Vitamins Folic acid Aqueous

Date

01.01.2015

Test Name

Data Path File

2015\January\Vitamins\01.01.2015

Matrix

Raw-Data File Re TUV/BLR/F-360

Equiment

Analyst

LC-MS/MS 6460A (BLR/PS-CRS/006.03

Manjunath K/Prasad BKVR

With Multilevel Calibration

Concentration (ug/kg)

-20000.00 0.00 20000.00 40000.00 60000.00 80000.00 100000.00 120000.00 140000.00

Calibration Standards

Area-Analyte

y = 638.88x - 1828.6 R² = 0.9985

Solvent Standard Level-1 Solvent Standard Level-2 Solvent Standard Level-3 Solvent Standard Level-4 Solvent Standard Level-5 Solvent Standard Level-6

2.00 5.00

1113.31 2659.72 10952.16 27674.14 59896.08 127588.73

20.00 50.00 100.00 200.00

R 2 ≥ 0.99

0.00

50.00 100.00 150.00 200.00 250.00

Criteria

Conclusion

Complies

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