SPIFAN Nutrients ERP Book_9-29-15

Carn-06 - Validation Report FOR ERP USE ONLY DO NOT DISTRIBUTE

day 15 day 16

338,625 332,365

342,389 340,596

day 15 day 16

2 681,014 340,507

7,083848

2 672,961 336,4805 33,8746805

Average of all days

347,38 mg/kg

ANAVA Source of Variation Between Groups

SS

df

MS

F

P-value

F crit

3354,811135 15 223,6540757 5,188242953 0,001098565 2,352222763

Within Groups

689,7258365 16 43,10786478

Total

4044,536971 31

Appendix 3: Limit of detection / quantification

Carnitine:

Replicate

9,414 ng/g 9,379 ng/g 9,145 ng/g 9,247 ng/g 9,418 ng/g 9,896 ng/g 9,409 ng/g 9,432 ng/g 9,810 ng/g 10,080 ng/g

Mean 9,523 ng/g Std 0,301 ng/g

LOD 0,9 ng/g LOQ 3,0 ng/g

In samples LOD 124 ng/g = 0,1 µg/g LOQ 413 ng/g = 0,4 µg/g