SPIFAN Nutrients ERP Book_9-29-15

GOS-02 SLV FOR ERP USE ONLY DO NOT DISTRIBUTE

Single Lab Validation Report for GOS in Infant Formula and Adult Nutritionals

Residue Plot Lactose Linear

16

14

12

10

8

6

4

2

0

0.00

5.00

10.00

15.00

20.00

-2

-4

% deviation from actual concentration

-6

Lactose Concentration (µg/mL)

Figure 4: Residue plot of the deviation from the actual concentration by linear calibration for lactose

Therefore, a quadratic fit was applied for both components (Figure 5 and 6).

Quadratic Calibration Curve Galactose

2

1.8

y = -0.0004x 2 + 0.1018x + 0.0034 R² = 1

1.6

1.4

1.2

1

0.8

0.6

Ratio Area/Area IS

0.4

0.2

0

0.00

5.00

10.00

15.00

20.00

Galactose Concentration (µg/mL)

Figure 5: Quadratic calibration curve for galactose

8